|[List of appearances]|
- “There is nothing that will hold me back!”
- ―Zane before he attacks the Treehorn Queen and reaches his true potential.
The Treehorn Queen is a large Treehorn, having more eyes, and is bigger. She was defeated by Zane, after discovering he was a robot, or a Nindroid, according to Jay. While unlocking his full potential, he suddenly lit up blue, and froze the Treehorn Queen. It is unknown what happened to the Queen after she was frozen. The other Treehorns fled.
- When attacking the Queen, Zane's eyes lit up light blue in the beam of light around him while unleashing his full potenial.
- The Treehorn Queen is only shown in the episode "Tick Tock".
- She has a different appearance than other Treehorns.
- Her legs are more spider like.
- She also has a wider face with several eyes.